Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis
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چکیده
منابع مشابه
Cholinergic action on the heart of the leech, Hirudo medicinalis.
Experiments were performed to determine the role of acetylcholine (ACh) in neuromuscular transmission in the heart of the leech Hirudo medicinalis. Superfused or iontophoretically applied ACh rapidly depolarized both isolated heart muscle cells and muscle cells in isolated hearts in a dose-dependent manner. the depolarization was associated with a conductance increase of the muscle membrane tha...
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Giant viruses and amoebae are common in freshwater, where they can coexist with other living multicellular organisms. We screened leeches from the species Hirudo medicinalis for giant viruses. We analyzed five H. medicinalis obtained from Tunisia (3) and France (2). The leeches were decontaminated and then dissected to remove internal parts for co-culture with Acanthamoeba polyphaga. The genome...
متن کاملA Study of Fibrogenesis in the Leech, Hirudo medicinalis
The fibrocytes and the fibres of Hirudo medicinalis have been studied with the electron microscope. The fibrocyte can be shown by other methods to contain triglyceride droplets, phospholipid droplets, and mitochondria, all of which can be recognized in the electron micrographs. In addition, a 'vesicular' component has been observed just below the cell surface. The collagenous 'cortex' of the fi...
متن کاملSpontaneous Electrical Activity and Behavior in the Leech Hirudo Medicinalis
In the absence of external stimuli, animals explore the environment by performing irregular movements, but the neuronal mechanisms underlying this arrhythmic motion are largely unknown. In this paper, we studied the relationship between the spontaneous neuronal activity in the leech (Hirudo medicinalis) and its behavior. We analyzed the electrical activity of isolated ganglia, chains of two con...
متن کاملCloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.
Cloned cDNAs have been isolated that encode a variant of hirudin, a potent thrombin inhibitor that is secreted by the salivary glands of the medicinal leech, Hirudo medicinalis. This variant probably corresponds to a form that has been purified from leech heads but differs in amino acid sequence from the hirudin purified from whole leeches. There are at least three hirudin transcripts detectabl...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-76715-w